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KMID : 0644120080210020038
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Journal of Oriental Gynecology
2008 Volume.21 No. 2 p.38 ~ p.48
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Effects of Hominis Placenta on the Growth of Human Uterine Myoma Cells and Cell Apoptosis
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Wee Hyo-Sun
Lee Chang-Hoon
Cho Jung-Hoon
Jang Jun-Bock
Lee Kyung-Sub
Lee Jin-Moo
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Abstract
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Purpose: This study was conducted to investigate the effects of Hominis Placenta (紫河車) on the growth of human uterine myoma cells and cell apoptosis.
Methods: Human uterine leiomyoma cells were cultured and treated with Hominis Placenta extract for 48 hours. Cell proliferation and activity was analyzed by MTT assay. We analyzed the cell cycle of human uterine myoma cells treated Hominis Placenta extract by FACS. Expression of proteins related to cell apoptosis (Bax, Bcl-2), cyclin-D1 and VEGF were evaluated by Western blotting method.
Results: The human uterine myoma cells treated by Hominis Placenta extract didn’t proliferate below the concentration of 10㎎/㎖. And there was no remarkable difference on cell cycle analysis below the concentration of 10㎎/㎖. The expression of Bax was decreased and the expression of Bcl-2 was increased after the treatment of Hominis Placenta extract. But the expressions of cyclin-D1 and VEGF were increased after the treatment of Hominis Placenta extract.
Conclusion: This study suggests that Hominis Placenta induce uterine myoma cell apoptosis and have effect on the myoma cell proliferation in the concentraion below 10㎎/㎖.
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KEYWORD
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Hominis Placenta, Uterine myoma cell, Cell Apoptosis
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